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KMID : 0352720120360030291
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Journal of Ginseng Research
2012 Volume.36 No. 3 p.291 ~ p.297
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Enzymatic Transformation of Ginsenoside Rb1 by Lactobacillus pentosus Strain 6105 from Kimchi
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Kim Se-Hwa
Min Jin-Woo
Lin-Hu Quan
Lee Sung-young
Yang Dong-Uk
Yang Deok-Chun
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Abstract
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Ginsenoside (ginseng saponin), the principal component of ginseng, is responsible for the pharmacological and biological
activities of ginseng. We isolated lactic acid bacteria from Kimchi using esculin agar, to produce β-glucosidase. We focused on the bio-transformation of ginsenoside. Phylogenetic analysis was performed by comparing the 16S rRNA sequences. We identifi ed the strain as Lactobacillus (strain 6105). In order to determine the optimal conditions for enzyme activity, the crude enzyme was incubated with 1 mM ginsenoside Rb1to catalyse the reaction. A carbon substrate, such as cellobiose, lactose, and sucrose, resulted in the highest yields of β-glucosidase activity. Biotransformations of ginsenoside Rb1 were analyzed using TLC and HPLC. Our results confi rmed that the microbial enzyme of strain 6105 signifi cantly transformed ginsenoside as follows: Rb1→gypenoside XVII, Rd→F2 into compound K. Our results indicate that this is the best possible way to obtain specific ginsenosides using
microbial enzymes from 6105 culture.
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KEYWORD
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Panax ginseng, Lactobacillus pentosus, Transformation, Ginsenoside,
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